The Gene Expression Facility is dedicated to sample quality monitoring and analysis of gene expression at DNA, RNA and protein levels, routinely performing a multitude of biochemical and molecular biology techniques.
Consists of equipment for sample quality monitoring and gene expression analysis, including a Qubit 2.0 fluorometer (Thermo Fisher Scientific) and a NanoDrop 2000c spectrophotometer (Thermo Scientific), standard gel electrophoresis (Bio-Rad), Trans-Blot Turbo System (Bio-Rad), absorbance plate readers (Bio-Rad), Chemidoc MP and Chemidoc XRS gel/membrane and X-ray film imaging (Bio-Rad), end-point thermocyclers (Bio-Rad and Thermo Scientific), and real time PCR systems including the 7300 Real-Time PCR (Applied Biosystems) and sate-of-the-art QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). The later enables high-throughput, quantitative gene expression, combining 384-well microfluidic gene expression, predesigned or customed card arrays, with multiplexing (21 filter combinations), and fast real-time capabilities.
Additional equipment includes a Guava easycyte 5HT benchtop flow cytometer (Merck Millipore) for high-throughput cell analysis in 96-well format, and stopped-flow (HiTech Scientific) and patch-clamp setups (Axon Instruments) used for kinetic analyses of ion channel activity.
Provides technical support ranging from advice in experimental design to data analysis.
Head: Rui Castro