Margarida M. F. B. Silva

PhD (2002) in Pharmacy (Biochemistry), Universidade de Lisboa
Assistant Professor, Department of Biochemistry and Human Biology

Faculdade de Farmácia, Universidade de Lisboa — Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal

T (+351) 217 946 400
F (+351) 217 946 491
Researcher ID B-6476-2013
Group Researcher ID B-5293-2014
Scopus Author ID 24780063300

Research Interests

Margarida works on the elucidation of drug-induced actions in cellular processes for the improvement of therapeutic intervention in relevant diseases, mainly at the liver-brain axis. Her research has a strong focus on metabolome, studying the effects of small acid drugs on mitochondrial bioenergetics and on the modulation of protein acetylation through nuclear communication. Metabolite-targeted mass spectrometry (GC-MS, LC MS/MS) is used for analyses of biomarkers in biological samples and for the understanding of protein function and cell homeostasis.

Selected Publications

 

Moedas MF, Adam AAA, Farelo MA, IJlst L, Chamuleau RAFM, Hoekstra R, Wanders RJA, Silva MFB. Advances in methods for characterization of hepatic urea cycle enzymatic activity in HepaRG cells using UPLC-MS/MS. Analytical Biochemistry 2017; 535, 47-55.

Moedas MF, van Cruchten AG, IJlst L, Kulik W, Tavares de Almeida I, Diogo L, Wanders RJA and Silva MFB. Transient decrease of hepatic NAD+ and amino acid alterations during treatment with valproate: new insights on drug-induced effects in vivo using targeted MS-based metabolomics. Metabolomics 2016; 12:142.

Luís PB, Ruiter J, Ijlst L, Tavares de Almeida I, Duran M, Wanders RJA, Silva MFB. Valproyl-CoA inhibits the activity of ATP- and GTP-dependent succinate:CoA ligases. J Inherit Metab Dis. 2014;37: 353-357

Aires CC, van Cruchten A, Ijlst L, de Almeida IT, Duran M, Wanders RJ, Silva MFB. New insights on the mechanisms of valproate-induced hyperammonemia: inhibition of hepatic N acetylglutamate synthase activity by valproyl-CoA. J Hepatol 2011; 55: 426-434.

Silva MFB, Aires CC, Luís PB, Ruiter JP, Ijlst L, Duran M, Wanders RJA, Tavares de Almeida I. Valproic acid metabolism and its effects on mitochondrial fatty acid oxidation: a review. J Inherit Metab Dis 2008; 31: 205-216.