Margarida Maria Fernandes Baptista e Silva
PhD (2002) in Pharmacy (Biochemistry), Universidade de Lisboa
Assistant Professor, Biochemistry and Human Biology
Faculdade de Farmácia, Universidade de Lisboa — Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal
Pharmaco-toxicology motivates Margarida Silva and her students to investigate drug-induced actions and reactions in relevant diseases for the improvement of therapeutic approaches. Her projects focus on elucidating the effects of small acid drugs on mitochondrial metabolism, cell bioenergetics and on the modulation of protein acetylation. Metabolite-targeted mass spectrometry (GC-MS, LC-MS/MS) is used for analyses of biomarkers in biological samples and for the characterization of specific targets at the biochemical and molecular levels.
Moedas MF, van Cruchten AG, IJlst L, Kulik W, de Almeida IT, Diogo L, Wanders RJA and Silva MFB; Transient decrease of hepatic NAD+ and amino acid alterations during treatment with valproate: new insights on drug-induced effects in vivo using targeted MS-based metabolomics; Metabolomics, (2016) 12:142.
Luís PB, Ruiter J, Ijlst L, de Almeida IT, Duran M, Wanders RJ, Silva MFB. Valproyl-CoA inhibits the activity of ATP- and GTP-dependent succinate:CoA ligases. J Inherit Metab Dis. 2013; Oct 24. [Epub ahead of print].
Aires CC, van Cruchten A, Ijlst L, de Almeida IT, Duran M, Wanders RJ, Silva MFB. New insights on the mechanisms of valproate-induced hyperammonemia: inhibition of hepatic N acetylglutamate synthase activity by valproyl-CoA. J Hepatol 2011; 55:426-434.
Aires CC, Ijlst L, Stet F, Prip-Buus C, de Almeida IT, Duran M, Wanders RJ, Silva MFB; Inhibition of hepatic carnitine palmitoyl-transferase I (CPT IA) by valproyl-CoA as a possible mechanism of valproate-induced steatosis. Biochem Pharmacol 2010; 79:792-799.
Silva MFB, Aires CC, Luís PB, Ruiter JP, Ijlst L, Duran M, Wanders RJA, Tavares de Almeida I; Valproic acid metabolism and its effects on mitochondrial fatty acid oxidation: a review. J Inherit Metab Dis 2008; 31:205-216.