PhD (1994) in Pharmacy (Medicinal Chemistry), Universidade de Lisboa
Assistant Professor, Medicinal Chemistry and Organic Chemistry
Faculdade de Farmácia, Universidade de Lisboa — Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal
Constantino’s research interests are the study of xenobiotic metabolism and the development of new drug delivery systems. His PhD was in the mechanism of amide metabolism by CYP 450 and later he studied drug metabolism. From that he got interested in prodrugs, specifically in their activation by metabolic reactions and in the prevention of prodrug inactivation. Currently he is studying ester prodrugs for the treatment of tuberculosis. He is also an expert in patents in the area of Pharmacy.
Pires D, Valente E, Simões, MF Carmo N, Testa B, Constantino L Anes E. Esters of pyrazinoic acid are active against pyrazinamide-resistant strains of Mycobacterium tuberculosis and other naturally resistant mycobacteria in vitro and ex vivo within macrophages. Antimicrobial Agents and Chemotherapy 2015; 59:7693–7699.
Prigol M, Nogueira C W, Zeni G, Bronze MR , Constantino L. In vitro metabolism of diphenyl diselenide in rat liver fractions. Reaction with GSH and binding to thiol groups. Chem Biol Interact 2012; 200:65-72.
Mata G, do Rosario VE, Iley J, Constantino L, Moreira R. A carbamate-based approach to primaquine prodrugs: Antimalarial activity, chemical stability and enzymatic activation. Bioorg Med Chem 2012; 20: 886-892.
Valente E, Simões MF, Testa B, Constantino L. Development of a method to investigate the hydrolysis of xenobiotic esters by a Mycobacterium smegmatis homogenate. J Microbiol Methods 2011; 85: 98-102.
Simões, M F , Valente E, Anes E, Constantino L Lipophilic POA Amide and Ester Prodrugs. Stability, Activation and activity against M. tuberculosis. Eur J Pharm Sci 2009; 28: 257-63.