Joana Paiva Gomes Miranda
University degree in Microbiology and Genetics (2000), Faculdade de Ciências da Universidade de Lisboa (FCUL)
PhD in Biochemistry (2006), Instituto de Tecnologia Química e Biológia/Universidade Nova de Lisboa
Faculdade de Farmácia, Universidade de Lisboa — Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal
Joana Miranda works in the field of tissue engineering and alternative methods for drug testing. Her research focus on the development of 3D in vitro cultures of hepatocytes or human MSCs for in vitro toxicology and cell therapy applications. In particular, 3D hepatocytes and human HLC cultures are used for drug safety and biotransformation studies. In addition, aggregation strategies of MSCs are also employed for priming cells towards an enhanced potential for cellular therapies (e.g. skin regeneration, rheumatoid arthritis).
Cipriano M, Freyer N, Knöspel F, Oliveira NG, Barcia RN, Cruz H,Cruz P, Castro M, Santos JM, Zeilinger K, Miranda JP. 2016. Self-Assembled 3D Spheroids and Hollow-fibre Bioreactors improve MSC-Derived Hepatocyte-Like Cell Maturation In Vitro. Arch Toxicol. 2016 Sep 2. [Epub ahead of print]
Pinheiro PF, Pereira SA, Harjivan SG, Martins IL, Marinho AT, Cipriano M, Jacob CC, Oliveira NG, Castro MF, Marques M, Antunes AMM*, Miranda JP. 2016. Hepatocyte spheroids as a competent in vitro system for drug biotransformation studies: nevirapine as a bioactivation case study. Arch Toxicol. 2016 Jul 14 [Epub ahead of print]
Santos JM, Camões SP, Filipe E, Cipriano M, Barcia R, Filipe M, Teixeira M, Mosqueira D, Nascimento DS, Simões S, Gaspar M, Pinto-do-Ó P, Cruz P, Cruz H, Castro M, Miranda JP. 3D spheroid cell culture of umbilical cord tissue-derived MSCs (UCX®) leads to enhanced paracrine induction of wound healing. Stem Cell Res Ther.2015. 6(1): 90-109
Miranda JP, Filipe E, Fernandes AS, Almeida J, Martins JP, Barcia R, Cruz P, De la Fuente A, Abal M, Castro M, Cruz H, Santos JM. The human umbilical cord tissue-derived MSC population UCX® promotes early motogenic effects on keratinocytes and fibroblasts and G-CSF-mediated mobilization of BM-MSCs when transplanted in vivo. Cell Transplant. 2015. 24(5): 865-77.
Miranda JP, Rodrigues A, Tostões RM, Leite S, Zimmerman H, Carrondo MJ, Alves PM. Extending Hepatocyte Functionality for Drug-Testing Applications Using High-Viscosity Alginate-Encapsulated Three-Dimensional Cultures in Bioreactors. Tissue Eng Part C Methods 2010; 16(6):1223-32