Joana P. Miranda
PhD (2006) in Biochemistry, ITQB/Universidade NOVA de Lisboa
Assistant Researcher (FCTinvestigator)
Faculdade de Farmácia, Universidade de Lisboa — Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal
Joana Miranda works in the field of tissue engineering and alternative methods for drug testing. Her research focuses on the development of 3D in vitro cultures of hepatocytes or human MSCs for in vitro toxicology and cell therapy applications. In particular, 3D hepatocytes and human HLC cultures are used for drug safety and biotransformation studies. In addition, aggregation strategies of MSCs are also employed for priming cells towards an enhanced potential for cellular therapies (e.g. skin regeneration, rheumatoid arthritis).
Cipriano M, Correia JC, Camões SP, Oliveira NG, Cruz P, Cruz H, Castro M, Ruas JL, Santos JM, Miranda JP. The role of epigenetic modifiers in extended cultures of functional hepatocyte-like cells derived from human neonatal mesenchymal stem cells. Arch Toxicol 2017; 91: 2469-2489.
Cipriano M, Freyer N, Knöspel F, Oliveira NG, Barcia R, Cruz PE, Cruz H, Castro M, Santos JM, Zeilinger K, Miranda JP. Self-assembled 3D spheroids and hollow-fibre bioreactors improve MSC-derived hepatocyte-like cell maturation in vitro. Arch Toxicol 2017; 91: 1815-1832.
Pinheiro PF, Pereira SA, Harjivan SG, Martins IL, Marinho AT, Cipriano M, Jacob CC, Oliveira NG, Castro MF, Marques MM, Antunes AM, Miranda JP. Hepatocyte spheroids as a competent in vitro system for drug biotransformation studies: nevirapine as a bioactivation case study. Arch Toxicol.2017; 91: 1199-1211.
Méndez-Giménez L, Becerril S, Camões SP, Silva IV, Rodrigues C, Moncada R, Valentí V, Catalán V, Gómez-Ambrosi J, Miranda JP, Soveral G, Frühbeck G, Rodriguez A. 2017. Role of aquaporin-7 in ghrelin- and GLP-1-induced improvement of pancreatic β-cell function after sleeve gastrectomy in obese rats. Int J Obes (Lond) 2017; 41: 1394-1402.
Santos JM, Camões SP, Filipe E, Cipriano M, Barcia R, Filipe M, Teixeira M, Mosqueira D, Nascimento DS, Simões S, Gaspar M, Pinto-do-Ó P, Cruz P, Cruz H, Castro M, Miranda JP. 3D spheroid cell culture of umbilical cord tissue-derived MSCs (UCX®) leads to enhanced paracrine induction of wound healing. Stem Cell Res Ther2015; 6: 90-109.