António J. I. Alfaia
PhD (2007) in Pharmacy (Pharmaceutical Chemistry), Universidade de Lisboa
Assistant Professor, Therapeutics and Pharmaceutical Chemistry
Faculdade de Farmácia, Universidade de Lisboa — Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal
Currently I’m integrated in the Biotechnology and Bioprocess Development cluster of CBT, from iMed.ULisboa.
My knowledge on physical, chemical and biological processes is used in emerging biotechnology approaches for the bioproduction of drugs, pointing to:
i. Design and develop value-added bioactive compounds (e.g. peptides, glycosides); ii. Design and modulation of improved bioconversion processes; iii. Modulation enzyme activity and stability by High Pressure and High Temperature technology.
Furtado A, Rosário PM, Calado ART, Alfaia AJI, Ribeiro MH, , High pressure studies on hesperitin production with hesperidinase free and immobilized in calcium alginate beads. High Pressure Research 2012; 32: 128-137.
Vila-Real H, Alfaia AJI, Rosa JN, Gois PMP, Rosa ME, Calado ART, Ribeiro MHL. Alfa-rhamnosidase and beta-glucosidase expressed by naringinase immobilized within new ionic liquid sol-gel matrices: activity and stability studies. J Biotechnol 2011; 4: 147-158.
Vila-Real H, Alfaia AJI, Calado ART, Phillips, RS, Ribeiro MHL.. High pressure: a tool to improve the enzymatic production of glycosides. High Pressure Research 2011; 3: 475-487.
Ribeiro M, Afonso C, Vila-Real H, Alfaia AJI, Ferreira L. Contribution of response surface methodology to the modeling of naringin hydrolysis by naringinase Ca-alginate beads under high pressure. LWT-Food Science and Technology 2010; 43: 482-487.
Alfaia AJI, Calado ART, Reis JCR. Quaternization reaction of aromatic heterocyclic imines in methanol – a case of strong anti-reactivity selectivity principle with isoselective temperature. Eur J Org Chem 2000; 21: 3627-3631.